Bca protein assay kit takara

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August undefined, 2024

WebTotal Protein Assay Kits PI23209 Thermo Scientific™ Pierce™ Bovine Serum Albumin Standard Ampules, 2 mg/mL High-quality, calibrated BSA solutions (2mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration. Supplier: Thermo Scientific™ 23209 Catalog No. PI23209 $112.40 / Pack … WebMar 27, 2024 · Catalog Number: T9300A. Info: Supplier Page. I use this kit to quantify protein lysates from mainly 24, 12 and 6-well plates. We have been using it for 2 years, …

Is there an expiration date for Pierce BCA Protein assay

WebA protein assay can be set up to measure the concentration of the unknown protein sample (mg/ml), or it can be set up to determine the total amount of protein in the unknown protein sample (mg). The BCA assay has a linear concentration range between 200–1,000 µg of protein per milliliter. In the standard assay, only 0.1 ml protein sample is ... WebBCA Protein Assay (Thermo Scientific, #23225) Clear, flat-bottom 96-well plate (such as Falcon, #353072) ... (BSA) standard provided by the kit (comes at 2 mg/mL). This can be accomplished as follows: Pipette 55 µL of BSA standard into tube 1 … lake cressbrook address

[생명과학실험] Protein quantification : BCA assay : 네이버 블로그

WebMay 1, 2024 · The protein concentration was determined by a BCA Protein Assay Kit (TaKaRa, Beijing, China). The relative GSH contents were determined to be (2*GSH-2*GSSG)/Protein. Cellular localization 2 × 10 4 B16 cells were seeded on a … WebJul 25, 2016 · The protein concentration of the supernatant was determined by the Enhanced BCA Protein Assay Kit (Beyotime, Jiangsu, China). ... (TaKaRa Biotechnology, Dalian, China) to synthesize cDNA. qPCR was performed on a Bio-Rad CFX-96 Connect Real-Time System (Bio-Rad, Foster City, CA, USA) using iTaqTM Universal SYBR … WebProtein concentration in the cell lysates was determined using the BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of whole protein extracts were separated on 10% SDS-PAGE gels for detecting expression of ECM1 and internal control β-actin, respectively. After transferred to polyvinyl difluoride membranes, samples were blocked ... helice 10 pas

Pierce™ BCA Protein Assay Kit - insecc.org

TaKaRa BCA Protein Assay Kit

WebOct 3, 2024 · The BCA protein assay is used to quantify total protein in a biological sample. BCA stands for Bicinchoninic acid, which is the key reagent used to produce a … lake crescent 华盛顿州WebTaKaRa BCA Protein Assay Kit (Cat. # T9300A) NOTE : This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do not use this product as food, cosmetic, or household item, etc. Takara products may not be resold or transferred, modified for resale or transfer, or used lake crescent olympic park

"WebFor western blotting, total protein was extracted with radioimmunoprecipitation buffer, and total protein concentration was measured using the BCA protein assay kit (Beyotime). The extracted total protein was separated using 12.5% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (ACE Biotechnology) and transferred onto a ... " - Bca protein assay kit takara

Bca protein assay kit takara

WebApr 10, 2024 · The digested genome was self-ligated using the Takara DNA Ligation Kit Ver 1 (Takara Bio) and used as the PCR template. Primers were designed by referencing the partial V-BPO sequence obtained from the degenerate PCR in (Partial cloning of LsVBPO1 by degenerate PCR) (pair 2 in Table S1). Takara Ex Taq was used under the following … WebPierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible protein assay for determination of protein …

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WebMar 1, 2024 · 本制品是一种对蛋白质溶液进行高灵敏度比色定量的试剂盒，可用于含有表面活性剂蛋白质溶液的定量。 BCA蛋白质定量包括两步反应，首先，二价铜离子 (Cu2+) 在碱性条件下被蛋白质的肽键还原成一价铜离子 (Cu+) ，还原后的铜离子浓度与溶液中所含蛋白质浓度成正比。其次，两个分子的bicinchoninic acid (BCA) 络合一个一价铜离子 (Cu+) … WebBicinchoninic acid (BCA) assay or Smith assay is a copper-based colorimetric assay for total protein quantification. BCA rely on the formation of a Cu 2 + –protein complex in a basic environment, followed by reduction of the Cu 2 + to Cu + ( Smith et al., 1985 ).

WebNov 23, 2024 · The circRNA, mRNA, and miRNA were amplified using the SYBR Green PCR kit, also from Takara (Shanghai, China). Supplementary Table S1 represents the primer information. ... RIPA buffer was used to separate the total cellular protein, and then the BCA assay kit (Beyotime) was used to quantify the protein level. Following the … WebTaKaRa BCA Protein Assay Kit Bicinchoninic acid (BCA) 시약을 이용한 단백질 정량 kit 간단한 조작으로 단시간에 단백질 정량 가능 단백질 종류에 영향을 받지 않아 0.02~2 mg/㎖의 넓은 범위에서 단백질 정량 가능 계면 활성제에 의한 영향을 받지 않음 제품 설명 TaKaRa BCA Protein Assay Kit은 매우 높은 감도의 발색 반응 (colormetric)을 이용하여 …

WebThe BCA assay primarily relies on two reactions. First, the peptide bonds in protein reduce Cu 2+ ions from the copper (II) sulfate to Cu 1+ (a temperature dependent reaction). The amount of Cu 2+ reduced is proportional to the amount of protein present in the solution. WebThe BCA Protein Assay combines the protein-induced biuret reaction (see above) with the highly sensitive and selective colorimetric detection of the resulting cuprous cation (Cu1+) by bicinchoninic acid (BCA). Thus, two steps are involved. First is the biuret reaction, whose faint blue color results from the reduction of cupric ion to cuprous ion.

Weblimits of compatibility for a wide variety of substances are listed in the instructions for the BCA Protein Assay Kit (Product No. 23225 or 23227). Some substances, including most reducing agents, interfere at even low concentrations in the BCA Assay. Examples include 5 mM DTT (for the BCA and Micro BCA™ Assays) and 0.1 M Glycine (for the ...

WebMar 1, 2024 · 本制品是一种对蛋白质溶液进行高灵敏度比色定量的试剂盒，可用于含有表面活性剂蛋白质溶液的定量。. BCA蛋白质定量包括两步反应，首先，二价铜离子 (Cu2+) … lake crescent rental homesWebMar 8, 2016 · (BCA) Protein Assay (Cat. # 786 -570, 786 -571 , 786 -890, 786 -891 ) Page 2 of 8 ... The kit is shipped at ambient temperature. Upon arrival, store the protein standards at 4°C. The remaining kit components should be stored at … lakecrestWebDec 23, 2024 · 干货分享 BCA Protein Assay Kit常见问题和注意事项 ... ⑦不同蛋白用 BCA 试剂盒检测，都会有独特的吸光度反应。通常使用 BSA 作为标准定量未知蛋白的浓度，但如果需要精确定量，请使用高纯度目的蛋白做标准品。 lake crescent lodge winter