Ctsk osteoclast
WebSep 24, 2024 · CTSK–mGFP cells in the primary spongiosa morphologically consistent with osteoclasts stained negative for CD200 (bottom panels). Scale bar, 20 µm. Images in c – e are representative of 3 ... WebApr 13, 2024 · The NFATc1 is known as a master transcription regulator of osteoclast differentiation, and involved in the expression of tartrate-resistant acid phosphatase (TRAP), a typical marker protein for osteoclasts, as well as cathepsin K (CTSK), and matrix metalloproteinase-9 (MMP-9) [14, 15]. The TRAP, CTSK, and MMP-9 proteins are …
Ctsk osteoclast
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WebSep 2, 2024 · The multinucleated giant cells labeled with EGFP are osteoclasts. Most of the cells in the culture system failed to differentiate into mature osteoclasts. Green (EGFP), CTSK; red, actin; blue, DAPI. b The osteoclast differentiation trajectory estimated by pseudotime analysis using the scRNA-seq data obtained from the in vitro osteoclast … WebDec 21, 2024 · For example, cathepsin K (Ctsk) has been used as a specific osteoclast marker, and Cre driven by its promoter is widely utilized for osteoclast investigations. However, Ctsk-Cre expression has recently been identified in other cell types, such as osteocytes, periosteal stem cells, and tenocytes. To better understand Ctsk-Cre …
Web微信公众号骨生物学介绍:骨生物学研究团队,分享学术信息~;骨生物学进展:brd9介导的染色质重塑通过负反馈机制抑制破 ... WebThe protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein …
WebFeb 27, 2024 · The upregulation of Zeb1 expression was confirmed by real‐time PCR and coincides with that of other well‐known osteoclast maturation makers, including Acp5 and Ctsk (Fig 1C). Similarly, at the protein level, Zeb1 content increases as BMDMs are induced to form osteoclasts (Fig 1D; Appendix Fig S1A). WebApr 14, 2024 · Osteoporosis is a disease that is characterised by reduced bone mineral density (BMD) and can be exacerbated by the excessive bone resorption of osteoclasts …
WebBy contrast, Ctsk-targeted osteoclasts have been reported to maintain normal bone formation by multiple mechanisms, including elevated platelet-derived growth factor-BB and sphingosine 1-phosphate expression and protecting pro-osteoblastogenic factors from Ctsk-mediated proteolytic inactivation (77–80). Here, the double deficiency of Mmp9 and ...
WebJul 13, 2024 · Cathepsin K (Ctsk) is a cysteine protease of the papain superfamily initially identified in differentiated osteoclasts; it plays a critical role in degrading the bone … biolincc websiteWebMar 31, 2024 · The completion of the differentiation of multinucleated osteoclasts is the expression of mature markers, such as cathepsin K (CTSK), MMP9, and tartrate-resistant acid phosphatase (TRAP) . CTSK is a protease that can decompose gelatin, elastin, and collagen and it plays a role in decomposing cartilage and bone in bone tissue ( 69 , 70 ). bioline age beauty secretWebNational Center for Biotechnology Information daily mail hospitals bribedWebNo fluorescence signal could be observed under any conditions in Ctsk-Cre osteoclast cultures (Figures 4D–F). Taken together, the experiments shown in Figure 4 indicate that our fluorescence-based Ctsk reporter assay (FRAMCO1.1) is suitable for the long-term follow-up and quantification of osteoclast-specific gene expression. Analysis of ... bioline alpha-select bio-85026WebNov 9, 2024 · Although letrozole suppressed the fusion process of osteoclasts, it remained unclear even when it also suppressed the direct osteoclastogenic pathway. Interestingly, letrozole did not affect the expression of NFATc1 and c-Fos but reduced the expression levels of CTR, TRAP, and CTSK, the osteoclast differentiation markers. biolin brain pillsWebApr 14, 2024 · Osteoporosis is a disease that is characterised by reduced bone mineral density (BMD) and can be exacerbated by the excessive bone resorption of osteoclasts (OCs). Bioinformatic methods, including functional enrichment and network analysis, can provide information about the underlying molecular mechanisms that participate in the … daily mail horoscopes 2023WebКомпьютерная томография височных костей: что показывает, как делается, показания ... daily mail horse racing page