Ea. hy926 cells
WebMay 15, 2024 · EA.hy926 endothelial cells were seeded at a density of 9000 cells/cm 2 on non-coated (Panel A), Matrigel-coated (Panel B) or collagen-coated (Panel C) 96 well … WebJul 27, 2024 · Human umbilical vein endothelial cells (HUVECs, Ea.hy926) were used to observe the cells adhesion to the samples. Firstly, the sterilized samples were put into 24-well culture plates, then the endothelial cells were seeded on the samples under a concentration of 2 × 10 4 cells/mL. After a 24 h incubation period, the samples were …
Ea. hy926 cells
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WebA permanent human cell line (EA.hy926) preserves the characteristics of endothelin converting enzyme from primary human umbilical vein endothelial cells. 1 reference. In … WebTo cite this cell line use: EA.hy 926 (RRID:CVCL_3901) Comments Group: Space-flown cell line (cellonaut). Characteristics: Produced by the fusion of A549/8 with human umbilical vein endothelial cells. Doubling time: 12 hours (PubMed= 6407019 ). Omics: Transcriptome analysis by microarray.
WebApr 7, 2024 · EA.hy926 cells were cultured in 96-well plates and were serum-starved for 12 h in DMEM with 0.2% FBS before the vehicle, ET-1, or plant extract was added for 24 h. The Annexin V FITC Assay Kit (Cayman Chemical) was used and analyzed using Spectra Max Gemini EM at 485/535 nm per manufacturer protocols to determine the percentage of … WebJun 9, 2024 · Serum-starved control and ATG7-KO-EA.hy926 cells had similar autophagosome accumulation at baseline. However, upon autophagosomal inhibition with CQ, EA.hy926 ATG7-KO showed significantly lower levels of autophagosomal fluorescence than controls treated with CQ, failing to fully maintain the autophagic flux ( Figure 2 A).
WebJun 27, 2013 · EA.hy 926 is a permanent cell line derived by fusing human umbilical vein endothelial cells with cell line A549 [ 23 ]. The culture was maintained according to the protocol provided by the distributor of the cell line … WebThe EAhy 926 cell is a hybridoma line derived from human endothelium and A549/8 cells. They display stable endothelial characteristics and may provide an indication of how endothelial cells respond to photodynamic therapy. Synonyms: EA.hy926 cell Tree view Term mappings - Graph view Reset tree Show all siblings experimental factor material …
WebOct 25, 2024 · Senescent EA.hy926 cells express a β-gal, which was detected using a Beta Galactosidase (β-gal) Activity Assay kit (BioVison, Milpitas, CA, USA), according to the manufacturer's instructions, based on the formation of a local blue precipitate upon enzyme-mediated cleavage.
WebMar 31, 2024 · EA.hy926 cells, transfected with striatin siRNA or controls (untransfected), were treated with estrogen (5 × 10−8 mol/L), and mRNA levels of PTGIS (A) and PTGS1 (B) were determined. Results show no significant difference in the genomic response after 5 hours of estrogen stimulation in the transfected and control cells (*, P < .05 vs vehicle). dhrm alternate hiring processWebEA.hy926 cells were treated with these gene complexes in the culture solution. EA.hy926 cells were treated with pEGFP-ZNF580 as a negative control group, and cells were treated with PEI 10 kDa/pDNA as a positive control group. The expression of GFP in transfected cells was observed under an inverted fluorescent microscope. cincinnati and michiganWebAug 5, 2015 · Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-dephenyltetrazolium bromide (MTT) assay, while the effect of TDD on the transcription profile of EA.hy926 cells subjected to H2O2-induced oxidative injury was evaluated by microarray analysis. Several signaling pathways, including apoptosis, were significantly … dhrm annual leave payoutWebEA.hy926 cell line (7), which mai- n tains an extensively differentiated ph-e notype (20), was also found to be r- e fractory to transfection, despite its vigorous growth properties. After opt-i mization of 5 transfection methods for EA.hy926 cells, Teifel et al. (23) found that the best transfection efficiency achieved was 2%. cincinnati and northern kentucky airportWebApr 13, 2024 · EA.hy926 cells within the logarithmic growth phase were seeded in 96-well plates (1 × 10 4 cells/cm 2) and incubated with different concentrations of phenolic acid (1, 5, or 25 μM) for 1 h. They were then separately co-incubated with TNF-α (10 ng/mL) or without TNF-α for 24 h. ELISA kits (Abcam, Cambridge, UK) were used to determine the ... dhrm alternative hiring processWebThe human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to … dhr madison county alabamaWebA permanent human cell line (EA.hy926) preserves the characteristics of endothelin converting enzyme from primary human umbilical vein endothelial cells. Life Sci. … dhr manufacturing services