Granuphilin c2a

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August undefined, 2024

NMR analysis of the granuphilin-C2A domain interacting with PI(4,5)P2-nanodisc demonstrates that, besides residues within the concave groove that are responsible for PI(4,5)P2 head group binding, the strand β5 was also affected. This region may be involved in direct binding to membrane and/or undergo conformation changes upon membrane binding. WebDec 12, 2013 · Proteins containing C2 domains are the sensors for Ca 2+ and PI(4,5)P 2 in a myriad of secretory pathways. Here, the use of a free-mounting system has enabled us to capture an intermediate state of Ca 2+ binding to the C2A domain of rabphilin 3A that suggests a different mechanism of ion interaction.Structural comparisons between C2A …

Insights into the molecular recognition of the granuphilin …

WebJan 13, 2015 · Another example is the study of the interaction of granuphilin-C2A domain with phosphatidylinositol 4, 5-bisphosphate [PI(4, 5)P 2 ] head group and PI(4, 5)P 2 … WebOct 12, 2006 · Like granuphilin toward insulin granules, exophilin4 promotes the targeting of glucagon granules to the plasma membrane. Although the interaction of granuphilin with syntaxin-1a is critical for the targeting activity, exophilin4 does this primarily through the afﬁnity of its C2A domain toward the plasma membrane how bear got his short tail

A surprising modification lowers the lipid binding affinity of a ...

Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the … WebMay 29, 2024 · Multivalent lipid targeting by the calcium-independent C2A domain of Slp-4/granuphilin Aml A Alnaas 1, Abena Watson-Siriboe 1, Sherleen Tran , Mikias Negussie, Jack A. Henderson , J. Ryan Osterberg 1, Nara Lee Chon , Julianna Oviedo,1 Tatyana Lyakhova1, Cole Michel2, Nichole Reisdorph 2, Richard Reisdorph , Colin T. Shearn3, … WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. Slp-4 binds vesicular Rab proteins via an N-terminal … how many months until november 4th 2022

Multivalent lipid targeting by the calcium-independent C2A …

WebGranuphilin C2A displays a lateral diffusion constant comparable to other C2 domains. However, the diffusion of the granuphilin C2AB tandem on the same membrane appears to be slower; suggesting substantial PS contacts for the C2B domain within the C2AB tandem. This effect represents a weak WebDec 20, 2006 · Instead, the C2 domains, particularly the C2A domain, of exophilin4 showed markedly higher phospholipid-binding activities than those of granuphilin. The … how many months until november 23rdWebSep 1, 2014 · The granuphilin C2A domain shows significant protein-to-membrane FRET when added to liposomes closely resembling the lipid composition of the plasma … how bears make money

"WebApr 1, 2016 · Furthermore, granuphilin forms a 180-nm cluster at the site of each docked granule, along with granuphilin-interacting Rab27a and Munc18-1 clusters. These … " - Granuphilin c2a

Granuphilin c2a

WebCorvallis Veterinary Hospital. Jul 2007 - Sep 20092 years 3 months. 1543 NW 9th St. Corvallis, OR 97330. As part of a small veterinary clinic I gained proficiency in a diverse set of skills ... WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. ...

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WebLearn about Equinix DC2 carrier-neutral data center, located at 21715 Filigree Court, Ashburn, VA. See our interconnection options, certifications and more. WebNov 5, 1990 · Granuphilin, a tandem C2 domain containing protein, mediates the docking of insulin granules onto plasma membrane. The C2A domain plays key roles in this process through interaction with PI(4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A domain to PI(4,5)P2 head group, and further …

WebHHMI’s Janelia Research Campus in Ashburn, Virginia, cracks open scientific fields by breaking through technical and intellectual barriers. Our integrated teams of lab scientists … WebGranuphilin is a membrane binding protein responsible, in part, for docking insulin secretory vesicles to the plasma membrane. Granuphilin, also known as synaptotagmin …

WebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this study was to determine membrane-binding mechanisms, affinities, and kinetics of both granuphilin C2 domains using fluorescence spectroscopic techniques. WebGranuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this …

Webusing site-directed mutagenesis and NMR. Overall, granuphilin demonstrates the capability to bind to background anionic lipids and phosphoinositides inde-pendently, but the presence of both lipids greatly increases the binding afﬁnity of this protein. This suggests that granuphilin may serve as a coincidence de-

WebRab27a and Rab27b have recently been recognized to play versatile roles in regulating the exocytosis of secretory granules and lysosome-related organelles by using multiple effector proteins. However, the precise roles of these effector proteins in particular cell types largely remain uncharacterized, except for those in pancreatic β cells and in melanocytes. Here, … how bears make playoffsWebJan 1, 2024 · The results of an in vitro binding study indicated that the C2A domain of Slp3 and Slp5 functions as an atypical Ca 2+-dependent phospholipid-binding domain ... Slp4/granuphilin was originally identified as a rabphilin-like protein associated with insulin granules in pancreatic β cells (Wang et al. 1999). how bears liveWeband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the cluster contains other basic residues that impart a positively charged electrostatic surface (Fig. 1). The Slp-4 C2A domain plays a critical role in targeting its how bear spray worksWebAll Slp members basically share an N-terminal Slp homology domain (SHD) and C-terminal tandem C2 domains (named the C2A domain and the C2B domain) with the SHD and C2 domains being separated by a linker sequence of various length. Slp1/JFC1 and Slp2/exophilin 4 promote granule docking to the plasma membrane. Additionally, their … how beatable is breast cancerWebNov 25, 2005 · Deletion analysis showed that the syntaxin-2/3 (or Munc18-1/2)-binding site is a linker domain of Slp4-a (amino acid residues 144-354), a previously uncharacterized region located between the N-terminal Rab27A binding domain and the C2A domain. We also found that the Slp4-a.syntaxin-2 complex is actually present in rat parotid glands … how many months until november 30 2027WebThe C2A domain plays key roles in this process through interaction with PI (4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A … how bears mateWebOct 31, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of … how many months until november 1