NettetAll lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution Lane 1 : induced bacterially expressed human ORF2 Lane 2 : uninduced bacterially expressed human … NettetAnti-LINE1 ORF2 Antibody, clone 1G4E11 Anti-LINE1 ORF2, clone 1G4E11, Cat. No. MABE1987, is a mouse monoclonal antibody that detects LINE-1 retrotransposable …

Development of a monoclonal antibody specific to the ... - PubMed

NettetORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons. Limitations NettetContact Cusabio to Customize LINE-1 retrotransposable element ORF2 Antibody, which reacts with Homo sapiens (Human) and will validated for ELISA, WB (ensure identification of antigen). Call: 301-363-4651 layflat downpipe

LINE-1 (L1, Long Interspersed Element, Retrotransposon ORF2) …

Nettet1. aug. 1998 · Heterologous expression of human LINE-1 ORF2 in yeast yielded a single polypeptide (M r 145 000) which reacted with specific antibodies and co-purified with a reverse transcriptase activity not present in the host cells. Various deletion derivatives of the ORF2 polypeptide were also synthesized. NettetAnti-LINE-1 Retrotransposable Element ORF2 Protein [1G4E11] Antibody. This mouse IgG1k monoclonal antibody was generated against bacterially-purified human … Nettet10. des. 2014 · L1 mobilization relies on the function of two, self-encoded proteins, ORF1 and ORF2. The ORF2 protein contains two characterized domains: endonuclease and reverse transcriptase. Results: Using a bacterially purified endonuclease domain of the human L1 ORF2 protein, we have generated a monoclonal antibody specific to the … layflat dishwasher