WebpEASY -T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products (3′-A overhangs). The vector is suitable with T7 RNA polymerase-mediated in vitro RNA … WebThis fragment contains 285 bp upstream of the Hsp-3start codon and was ligated into the T1 vector using the pEASY-T1 Cloning kit (Transgen) (Figure 1, step 2) to generate sufficient material for subsequent manipu- lations.
北京全式金生物(TransGen Biotech)_官方主页
WebpEASY ® - T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. ... •Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Storage Competent Cell at -70°C for six months; others at -20 ... WebDec 6, 2024 · The tmexCD1.2-toprJ1 and tmexCD2.2-toprJ2 gene clusters were amplified by PCR and ligated to the pEasy-T1 vector (Transgen, Beijing, China) using Gibson cloning (Gibson Assembly Cloning Kit, NEB). The primers used for subcloning are listed in Table S1 (available as Supplementary data at JAC Online). flixbus to new york
pEASY-T1 (linearized) Sequence and Map - SnapGene
WebpEASY® – T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY®- T1 Cloning vector. It is designed for cloning and sequencing Taq -amplified PCR products. … WebpEASY T1 载体 _生物学_自然科学_专业资料。 pEASYpEASY ?-T1 Cloning Kit 目录号:CT...测序用 M13 F,M13 R,T7 promoter 引物测序,进行 序列 分析。 注意! 1. 根据... pEASY -Blunt clonning vector 载体 说明书WORD版 pEASY -Blunt clonning vector 载体 说明书WORD版_生物学_自然科学_专业资料。 pEASY ...bases 3134-3807 2 pEASY -Blunt Restriction Map … WebConstruction of Clone Vectors pEASY-T1-2f PF4 and pEASY-T1-3f PF4. Source publication Polycistronic Expression of Human Platelet Factor 4 with Heparin-Neutralizing Activity in … flixbus to paris