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Phis-sumo vector

WebbIncludes: The Champion pET SUMO TA Cloning box contains linearized Champion pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. The SUMO Protease box contains SUMO Protease and buffers. Webbencoding amino acids 1-96 of SUMO-1 (lacking glycine 97 creating a SUMO-1 that is not susceptible to proteolytic removal by isopeptidases) was fused in frame to the amino …

Invitrogen™ Champion™ pET SUMO Expression System

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pETite N-His SUMO Kan vector map and sequence

http://www.biofeng.com/zaiti/dachang/pSUMO.html WebbThese vectors represent the combinatorial pairing of two different parent vector backbones with two different affinity tags. The target gene is cloned downstream of the … WebbCentrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. … philip whitmore kpmg

Characterization of the SAM domain of the PKD-related protein …

Category:SUMOylation of IQGAP1 promotes the development of

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Phis-sumo vector

SUMO-Tag Protein Expression & Purification - LifeSensors

Webbyeast 2-hybrid vectors (sundquist lab) pGADT7 and pGBKT7. Ligation Independent Cloning (LIC) vectors from Mark Connelly: pMCSG10, pMCSG7, pMCSG17-20, pMCSG19, … WebbFor two vectors and , the vector sum is obtained by placing them head to tail and drawing the vector from the free tail to the free head. What is the vector sum formula? To add or …

Phis-sumo vector

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Webb11 jan. 2016 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the above sequences into a pHis-SUMO vector (Senturia et al. … WebbWe have a modified pETM11 vector for the expression of SUMO3 fusion proteins available. This vector contains an optimized synthetic human sumo3 gene with appropiate restriction sites at the 3′-end of the sumo3 gene for the insertion of any gene of interest.. The small human SUMO3 protein (81 aa, 9.2 kDa) can be used as an N-terminal fusion partner for …

Webb7 juli 2014 · Hexahistidine small ubiquitin-like modifier (SUMO) tagged constructs were generated by cloning the ANKS3-SAM and ANKS6-SAM sequences into a pHis-SUMO … WebbSUMO-4V constructs were made to express active recombinant proteins ending in the terminal di-glycine residues (Fig. 1, additional C-terminal residues are cleaved normally in …

http://wolfson.huji.ac.il/purification/PDF/Tag_Protein_Purification/SUMO/LifeSensors_SUMOstarInsect.pdf Webb635690: pCMV-HA Vector Set. 635689. pCMV-Myc Vector Set. Each. USD $534.00. This vector set allows for cloning and expression of your gene of interest fused to a Myc tag at either the N- or C-terminus. Notice to purchaser. Our …

WebbOur SUMO-tag expression systems maximize the yield of soluble, functional proteins in E. coli. SUMO functions as both a chaperonin and initiator of protein folding, improving the …

Webb8 aug. 2024 · For the GST pull-down assay, full-length GI was cloned into the pHis-SUMO vector, and the sequence encoding the cytoplasmic tail of SOS1 (residues 453 to 1,146) … philip white hot sauceWebbsmall size of the pRham N-His SUMO Kan Vector (2.6 kb) facilitates cloning of large inserts and performing DNA manipulations, such as site- directed mutagenesis. The pRham N … try free mathWebbNovelMechanismofHeminCapturebyHbp2,the Hemoglobin-bindingHemophorefromListeria monocytogenes* … philip whitfield ukasWebbDownload PHIS database for standalone from PHISDetector webserver and check MD5 Due to the large size of the database file (~100G), we recommend using -c (continue) … try free minecraftWebb25 okt. 2012 · SUMO (smallubiquitinlikemodifier 是一种小分子泛素样修饰蛋白 ,广泛存在于各种真核细胞中,参与调节细胞凋亡、信号转导、RNA 转录、蛋白的核质运输以及细胞周 … philip whitfield \u0026 sons textiles ltdWebbsumo标签蛋白是一种小分子泛素相关修饰蛋白,是存在于真核生物中高度保守的参与蛋白质小泛素化相关修饰的一类大蛋白。 与GST、MBP或NusA相比,SUMO不仅可以作为重组 … philip whitfield hook nortonWebbThe best selection of Royalty Free Sumo Vector Art, Graphics and Stock Illustrations. Download 1,200+ Royalty Free Sumo Vector Images. philip whitmore